OptiPASS® Medium

OptiPASS® : Complete Serum-Free cell culture medium

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Optipass® Medium Applications for:

Available for either tumoral 2D cell culture…

Example with Triple-Negative Breast Cancer cell line MDA-MB-231

From Dubois et al. J. Clinical Medicine (2019)

…or for 2D bioproduction

(example with VERO E6 cell line)

For your monolayer / 2D cell cultures,
OptiPASS medium will allow you to:

Maintain cell proliferation and viability

Preserve cell phenotype

For adherent cells, preconized coating solutions are Matrigel®, Geltrex®, Fibronectin & Collagen 1

These tumoral cell lines can be cultured in

3D in OptiPASS medium (non-exhaustive)

Scanning Electron Microscopy (MEB) or Bright Field Imaging.

For your 3D cell cultures / Artificial tumors®, OptiPASS medium will allow you to:

Maintain or increase 3D cell culture proliferation rate

Maintain the metabolic heterogeneity / gradients within your culture (Live/Dead profiles)

Example with Triple-Negative Breast Cancer cell line MDA-MB-231

From Dubois et al. J. Clinical Medicine (2019)

Living cells (green) and dead cells (red)

Why using serum-free media for your cell cultures ?

Because you want to prevent the risk of serum contaminations and shortages

Because you want to stop using animal components for your cultures

Because you want to increase the reproducibility of your cultures 

OptiPASS® medium allows you to answer these problematics 

Another OptiPASS® application:

Reversible cell dormancy without freezing: a new conservation method

An alternative

to freezing

Only one step of

reconditioning

Similar spheroid

culture parameters

Similar response

to anticancer drugs

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