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OptiPASS® Medium

OptiPASS® : Complete Serum-Free cell culture medium

Adapted to 2D cell cultures of various cancer cell types. 

Adapted to improve and standardize artificial tumors®  reproducibility, conservation and growth.

Why using serum-free media for your cell cultures ?

Because you want to reduce the batch-to-batch variations inherent to the use of an
unstable and non reproducible component, such as Fetal Calf Serum

Because you want to prevent the risk of serum shortages & prion contaminations

Because you want to master / adapt the formulation of your culture medium

For your monolayer / 2D cell cultures,
OptiPASS medium will allow you to:

Maintain cell proliferation and viability

Preserve cell phenotype

For adherent cells, preconized coating solutions are Matrigel®, Geltrex®, Fibronectin & Collagen 1

Example with Triple-Negative Breast Cancer cell line MDA-MB-231

From Dubois et al. J. Clinical Medicine (2019)

For your 3D cell cultures / Artificial tumors®, OptiPASS medium will allow you to:

Maintain or increase 3D cell cultures proliferation rate

Maintain the metabolic heterogeneity / gradients within your culture (Live/Dead profiles)

Example with Triple-Negative Breast Cancer cell line MDA-MB-231

From Dubois et al. J. Clinical Medicine (2019)

Living cells (green) and dead cells (red)